How to check urine integrity with a Specimen Validity Test


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This Specimen Validity Testing device is for checking urine integrity for six different abnormalities before performing a drug test. These abnormalities may be the result of substitute urine or contamination due to donor abuse. It tests for adulterated compounds, dilution and pH levels and more.

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Test every sample with a Specimen Validity Test.

This device is used to identify abnormalities found in a person’s urine sample. These abnormalities may be the result of the donor adding a chemical to mask the outcome, dilution, and substitution. When used as directed, it establishes the integrity of human urine. The integrity has to be within standard parameters for the results of a drug test to work correctly. And remember a donor who is trying to cheat their test will do what is needed to pass.

Sample adulterating by substitution or adding adulterants is a prevalent practice. Testing their sample is paramount when analyzing a sample for an abused substance — the test checks for Oxidants, Specific Gravity, pH, Glutaraldehyde, Nitrites, and Creatinine.

The strip is designed to evaluate a donor’s urine sample comprehensively. Abnormalities found will alert you immediately. Only when the donor’s specimen is balanced and has the correct pH level, specific gravity, and their creatinine levels are normal will they pass.

What is adulteration?

Adulteration is the tampering of a urine sample with the intent of altering the result. The use of adulterants can cause false effects in drug tests by interfering with the screening process and by destroying the drugs present in the urine. Dilution may also be a tactic to produce false-negative results.

One of the best ways to check for adulteration is to determine specific urinary characteristics, as shown below:

  1. OX – Oxidants: Detects the presence of oxidants.
  2. S.G. – Specific Gravity: Checks for sample dilution.
  3. pH: Checks for the presence of acidic and alkaline adulterants.
  4. GLU – Glutaraldehyde: Checks for the presence of an exogenous aldehyde.
  5. NIT – Nitrites: Detects for commonly used commercial adulterants.
  6. CRE – Creatinine: Detects waste product called creatine present in human urine.

Directions for using the Specimen Validity Test:

  1. Remove a strip from the canister. Dip the strip into the urine specimen and remove it immediately.
  2. Blot the strip gently on its side to remove excess urine.
    Note:  For consistent results, blot the strip every time a new one used.
  3. Read the results at one (1) minute by comparing each pad with the color chart printed on the canister. DO NOT interpret after four (4) minutes.
  4. If the result indicates adulteration, refer to your supervisor or ask the donor to provide a fresh urine sample after a thirty (30) minute wait time.

specimen validity test chart

This is an example of the color comparison chart and test strip (right) included in the package.

NIH recommends the use of an Adulteratant Test and suggests that it checks for pH and specific gravity for initial and confirmatory results.

How can a sample be adulterated?

Adulterants placed in urine will cause false negatives either by interfering with the screening process or by eradicating the presence of drugs. Examples of products that interfere with a drug test include bleach, vinegar, Visine, sodium bicarbonate, sodium nitrite, Drano, soft drinks, and hydrogen peroxide.

Why should I check a donor’s urine with a Specimen Validity Test?

Using a Specimen Validity strip or adulterant check as it’s sometimes called is the most effective way to monitor urine integrity. We cannot express how important checking every sample before urine analysis. If you don’t, they win and pass the test. Proficient collector/administrators always use Specimen Tests to beat the cheaters and validate the sample is suitable for the drug test kit.

Documentation for using the Specimen Validity Test:

Click the link below to print instructions and color charts to aid you when testing urine samples.

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Additional information

Weight .125 lbs
Dimensions 6 × 1.5 × 1.5 in


Order notification.
We will always send an order confirmation via email. If you didnt receive a confirmation then double check you provided the correct email address. Next, check your email spam box and you may find the confirmation there. Because of the nature of the products we supply, often our emails will go into spam. If that is the case, move the email to your inbox and that will correct the problem so future updates arrive normally.

When will I get my order?
The arrival date depends on which shipping options were chosen, and how many items were in the order. Most orders are processed and shipped same day and take 3-5 days to arrive. Some products are shipped directly from the manufacturer and these usually take 5-7 working days to arrive.

Small packages are shipped via USPS Mail and take 2-5 days to arrive.

Shipping restrictions.
We do not ship orders to a P.O. Box, they must be shipped to a street address. You’ll be notified after placing your order if we’re unable to ship to the address you’ve specified.

NOTE: We do not ship orders to Alaska or Puerto Rico.

Screening cut-off levels. (Urine drug tests only.)

DrugIdentifierScreen cut-off
Amphetamines (AMP)1000 ng/ml
Barbiturates(BAR)300 ng/ml
Buprenorphine(BUP)10 ng/ml
Benzodiazepines(BZO)300 ng/ml
Cocaine 150(COC)150 ng/ml
Cocaine(COC)300 ng/ml
Ecstasy(MDMA)500 ng/ml
Marijuana(THC)50 ng/ml
Methamphetamine 500(mAMP)500 ng/ml
Methamphetamine(mAMP)1000 ng/ml
Methadone(MET)300 ng/ml
Morphine(MOP)300 ng/ml
Opiates(OPI)2000 ng/ml
Oxycodone(OXY)100 ng/ml
Phencyclidine(PCP)25 ng/ml
Propoxyphene(PPX)300 ng/ml
Tricyclic Antidepressants(TCA)1000 ng/ml
Note: Refer to manufacturers product insert for specific details.

Detection times. (Urine drug tests only.)

Opiates (OPI)Heroin1-2 days
Morphine2 days
Methadone3 days
Meperidine2-3 days
Codeine2 days
Propoxyphene6 hrs to 2 days
Oxycodone1-3 days
HallucinogensLysergic Acid1-5 days
Marijuana and CannabinoidsUp to 45 days
Phencyclidine1-4 weeks
StimulantsCocaine3-4 days
Amphetamines3-10 days
Methylene-2, 4 dioxy-methamphetamineUp to 24 hrs
Methamphetamine48 hrs to 10 days
Barbiturates (BAR)Pentobarbital2 days
Secobarbital2 days
Butabarbital2 days
Butalbital2 days
Phenobarbital1-3 weeks
Alcohol & MetabolitesEthanolUp to 1 day
MethanolUp to 1 day
IsopropanolUp to 1 day
Ethyl GlucuronideUp to 3 days
Ethylene GlycolUp to 1 day
Benzodiazepines (BZO)Diazepam5-7 days
Oxazepam5-7 days
Alprazolam5-7 days
Clonazepam5-14 days
Chlordiazepoxide5-7 days
Lorazepam5-7 days
Flunitrazepam72 hrs
Gamma-Hydroxybutyrate12 hrs
KetamineUp to 72 hrs


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